Leveraging Programmable CRISPR-Associated Transposases for Next-Generation Genome Engineering
Dr. Sam Sternberg discusses a novel CRISPR-Cas9 system using programmable, RNA-guided transposase, and highlights its implications for kilobase-scale genome engineering in cell and gene therapies.
Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal
Join Pilhan Kim, PhD for an in-depth discussion on the All-in-One intravital two-photon and confocal microscopy system.
Protocol Preview: In Vivo Gene Therapy Using Peptide-Based Delivery
Although gene therapy has been in discussion for decades, its translation to clinical practice has been a slow and challenging process. As a solution to this translational challenge, Allen et al. have proposed a peptide-based delivery method of Cre recombinase for in vivo gene therapy, which we review in this blog post.
The Cancer-Immunity Cycle: Methods, Challenges, and Opportunities
In this series, experts provide an overview of technologies, techniques, challenges, and emerging research questions along each step of the cancer immunity cycle.
#ExpertAnswers: Marta Walasek and Danielle Nguyen Truong on Stem Cells
Marta Walasek and Danielle Nguyen Truong provide tips and tricks to lock down effective genome editing conditions for CD34+ cells, methods to evaluate genome editing efficiency, and optimal culture conditions to maintain Hematopoeitic stem cell function and long-term editing effects.
Optimizing Cell Therapy Research Workflows
A 2-part mini series from STEMCELL Technologies highlighting optimized products, protocols, and workflows for cell therapy research.
Optimizing CD34+ Cell Genome Editing for Efficiency and HSPC Maintenance
Experts provide tips and tricks to lock down effective genome editing conditions for CD34+ cells, methods to evaluate genome editing efficiency, and optimal culture conditions to maintain HSPC function and long-term editing effects.
