Dr. Georgakopoulos started his PV loop research in 1994 alongside David Kass. Over the next 10 years together they worked on many “firsts” relating to PV Loop science, including development of the first systems and methodologies for obtaining PV loops in the mouse. In fact, they were the first to publish data showing PV loops collected in an animal model of this size. They went on to study force-frequency relations in the mouse model assessing calcium dynamics in vivo, and also studied the role of nitric oxide and neural control of the circulation through the baroreflex.