Dr. Cirrito started his PhD in Neuroscience at Washington University in the lab of Dr. David Holtzman (Neurology) studying Alzheimer’s disease in 1999. Much of his PhD work focused on the proteins and cellular pathways that eliminate amyloid-beta (Aβ) from the brain. Here he developed the first use of in vivo microdialysis to study Aβ kinetics in the brain of a living mouse. This was the first time someone was able to measure Aβ peptide longitudinally. In the Holtzman lab he realized the importance and value of collaboration, having worked with numerous departments across the university as well as outside groups and industries. John received his PhD in 2005, then became a post-doc in the lab of Dr. Steven Mennerick (Psychiatry) where he learned to pretend to be an electrophysiologist to study synaptic processes that regulate Aβ generation. He started his own laboratory (Neurology) at WashU studying Alzheimer’s disease in 2010, received tenure in 2015.