On November 5, 2014, InsideScientific hosted a webinar sponsored by TSE Systems discussing the challenge of behavioral phenotyping individual rodents within a social home cage environment. Speakers David P. Wolfer, Ewelina Knapska, and Holger Russig discussed the use of IntelliCage, a home-cage system able to conduct automated, high-throughput behavioral and cognitive testing. Automation technology minimizes the need for human intervention, reducing workload to the researcher while improving data reproducibility and animal welfare.
Several research applications are discussed, including profiling mice with hippocampal lesions based on spontaneous behaviors and spatial learning, characterizing appetitively and aversively motivated learning in MMP-9 knockout mice, and assessing cognitive rigidity in a valproic acid-induced mouse model of autism.
Follow the links below to access key educational points of the webinar…
Dr. Ewelina Knapska
- 39:46 Comparing classical behavioral tests to IntelliCage, and appetitively and aversively motivated learning in well-balanced conditions
- 46:03 Involvement of MMP-9 in appetitively and aversively motivated learning
- 52:15 Mouse models of autism and the assessment of cognitive rigidity
- 58:40 Dr. Ewelina Knapska summary
Dr. David P Wolfer
- 18:02 The 3 stages of mouse adaptation to the IntelliCage
- 18:50 Parameters measured during adaptation stages to characterize spontaneous behavior
- 21:37 Comparison of spontaneous behavior profiles in different strains
- 23:36 Effect of hippocampal lesions on spontaneous behavior profile and learning
- 28:07 Example battery of hippocampus-dependent spatial learning tasks
- 33:21 Tests to address motor impulsivity and reaction time
- 37:04 David P. Wolfer summary
- 60:04 Considering challenges relating to fighting and hierarchy, such as a dominant male controlling a limited resource such as sucrose, how is it possible to generate independent data in the IntelliCage?
- 62:33 How well do 129 mice learn in the IntelliCage?
- 63:38 Can the IntelliCage be used for rats?
- 64:42 With regards to the data you shared, can you comment on mice learning by observation?
- 68:43 How are shams measured together with different cohorts of mice, whether it’s in the same cage or in different cages?
- David P. Wolfer, MD
Institute of Anatomy, University of Zurich
University of Zurich profile
Google Scholar page
- Ewelina Knapska, PhD
Laboratory of Emotions Neurobiology, Nencki Institute of Experimental Biology
Network of European Neuroscience Institute profile
- HolgerRussig, PhD
Scientific Officer, TSE Systems Inc.
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