Experts provide an overview of ex vivo and in vitro methods, techniques, and model systems for research within the cancer-immunity cycle framework.
Immuno-oncology is a fast-growing branch of science, with an unprecedented number of immunotherapeutics being developed in recent years. It’s not without its challenges though, and researchers must often balance scalability with physiological relevance.
In this webinar, Dr. Rajendra Kumari and Dr. Gera Goverse discuss the basics of immuno-oncology and how cancer immunotherapies are studied along each step of the cancer immunity cycle. They provide an overview of in vivo, ex vivo and in vitro assays available, including both traditional as well as novel assays. They also describe the importance and applications of several technology platforms including flow cytometry, histology, single cell analysis, cytokine profiling, T cell cytotoxicity, and high content imaging, and highlight relevant examples from the literature that used them.
Key learning objectives:
- What is the cancer immunity cycle? How are the steps of the cycle regulated, and which steps are potential targets for immunotherapeutics?
- Understand the advantages and limitations of 2D, 3D, in vivo, and ex vivo models
- Applications of high-content imaging and why HCI analysis is so valuable for preclinical immuno-oncology research
Director of Immuno-Oncology