In this webinar, learn how to set up DC and CD8⁺ T cell co-culture experiments and assess T cell proliferation and activity.

Dendritic cells (DCs) are powerful antigen-presenting cells that process exogenous antigens and present them to CD4⁺ and CD8⁺ T cells to generate immune responses. The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules to CD8⁺ T cells is known as cross-presentation and is essential to induce antigen-specific responses of CD8⁺ T cells to fight infections and disease. Therefore, co-culturing DCs and CD8⁺ T cells is a valuable method for studying antigen presentation and T cell activation. Using co-cultured T cells, further studies can be conducted on the presentation of tumor antigens, T cell-mediated cytotoxicity, and memory T cell development. Such studies can help develop cancer treatments and vaccines that harness T cell functions to fight tumors and induce long-lasting immune memory for protection against cancer.

In this webinar, Dr. Catherine Ewen from STEMCELL Technologies will describe, in detail, how to set up DC and CD8⁺ T cell co-culture experiments that generate antigen-specific CD8⁺ T cells, and how to assess CD8⁺ T cell proliferation, functionality, and killing activity.

Key Topics Include:

  • Learn how to use DC/CD8⁺ T cell co-culture to study the antigen-specific responses of CD8⁺ T cells, including their activation, proliferation, cytokine production, and cytotoxic killing activity.
  • Explore the features of STEMCELL’s ImmunoCult™ Medium and Activators.
  • Isolate antigen-specific cells with EasySep™ Release.

Presenters

Catherine Ewen, PhD

Senior Scientist
Research & Development
STEMCELL Technologies

Dr. Catherine Ewen works as a Senior Scientist at STEMCELL Technologies, where she brings proof-of-concept products to market. She is passionate about scientific discovery and technological advancements, and her top goal has always been to build effective and collaborative teams that are driven by innovation and pursue scientific excellence.

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