Dr. Malmstrøm demonstrates how correlations between NMR data and bioactivity can be used to confirm the structure and activity of proteins.
An important aspect in analytical assessment of pharmaceutical proteins is the comprehensive characterization of their higher order structure (HOS). The most sensitive and detailed method for measuring HOS in proteins is unquestionably 2D NMR spectroscopy. It is well-established that the biological functions of proteins depend on their specific structures. However, the quality of bioactive proteins is traditionally assessed indirectly by cell-based potency assays. These assays usually take several days, are laborious, and have considerable variability. In addition, development of suitable assays for oral formulations is facing severe challenges due to excipient interference. The need for a rapid, precise, sensitive, and robust HOS assay has increased since the regulatory agencies are now approving “biosimilar” pharmaceutical proteins based on HOS similarities.
In this fifth and final event in the Bruker COVID-19 Webinar Series, Dr. Joan Malmstrom will demonstrate that NMR can be used to ensure that the structure, and thereby the activity, of a protein is intact. She will do this by focusing on her work that shows how to measure a correlation between bioactivity and NMR data. She will share specific examples of how this can be done by measuring the bioactivity and NMR data on intact samples as well as samples that have been stressed under extreme conditions.
CMC Analytical Support
Additional Content From Bruker BioSpin
Stimulating and Recording Neuronal Activity During Traditional fMRI, and the Benefits When Doing This at “Zero” Echo Time
Experts discuss electrical and optical-based techniques capable of stimulating and recording neuronal activity during fMRI, share examples using these tools to study neurovascular coupling, and introduce a method to acquire fMRI data at zero echo time and discuss the advantages over traditional fMRI technique.
Dr. Raanan Carmieli discusses the use of electron paramagnetic resonance (EPR) as a powerful technique for the study of transition metals, organic radical ions and transient radical-pairs in photoexcited systems.
Dr. Marty Pagel introduces the CEST MRI technique, demonstrates a basic experiment, and explains why measuring protein, acid, and metabolite content can be so beneficial in applications of brain ischemia and cancer.
A Comprehensive How-To Demonstration of Higher Throughput Excitation-Contraction Coupling Investigations
Join Michiel Helmes and Diederik Kuster as they demonstrate best practices and new techniques in high-content, higher throughput investigations of excitation-contraction coupling in isolated cardiomyocytes.
OBESITY SERIES 2020: Dr. Ania Jastreboff discusses the current use of anti-obesity pharmacotherapy, mechanisms involved, and agents in various stages of development with considerations for next steps.
Dr. Sharman explains the impact of using NMR for the quality control and release of drug molecules currently in shortage due to the Coronavirus pandemic.