Join Matt Borkowski and Dr. Tim West for this informative webinar covering the A-to-Z of assessing muscle performance and contractile function in single muscle fibers.
Assessing contractile function in single muscle fibers is an advanced experimental assay that can provide extremely useful data about muscle performance and the underlying mechanisms of muscle contraction commonly observed in basic physiology, molecular biology, cardiology and comparative physiology.
During this webinar sponsored by Aurora Scientific, Matt Borkowski will give an overview of the instrumentation and standard protocols used in elucidating the functionality of myofilament proteins to assess significant muscle fiber properties like power output, cross-bridge cycling and calcium sensitivity. Following, Dr. Tim West will cover how to isolate and prepare single permeabilized muscle fibers to properly utilize this technique to measure these properties, along with how to analyze the data obtained from the assay. This is an essential resource for anyone endeavouring to study muscle fiber mechanics.
Key Topics Include:
- Basic operating principles and theory of required instrumentation
- Understanding when and how to apply standard experimental protocols
- How to prepare samples for experimentation
- Analyzing real data and discussing the conclusions it provides
- Curve fitting procedures to determine peak power
Who Should Attend?
- Research Technicians interested in studying muscle function
- PIs and Lab Managers planning muscle function and related disease research
- Pharmaceutical researchers, lab managers and directors overseeing muscle function research projects
- Early career researchers focused on in vivo, in situ and ex vivo muscle function research
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Aurora Scientific Inc.
Chief Technician and Laboratory Manager
Royal Veterinary College