Oxygen influences multiple physiological parameters within cells. In addition to monitoring and controlling for CO2, humidity and temperature, scientists should consider the assessment of oxygen levels for in vitro studies. By removing a potential confounding factor, researchers can improve both scientific reproducibility and relevant outcomes.
This webinar hosted by Scintica Instrumentation builds on these concepts and discusses the importance of oxygen in cell culture. Dr. Nicky Pansters describes a better method for defining O2 concentration as well as options for monitoring and controlling oxygen levels. He also provides a brief overview of relevant scientific publications. Webinar participants will gain a better understanding of how accurate oxygen regulation will contribute to improved reproducibility of their experimental work, and what next steps to take to improve future research outputs.
Topics discussed in this webinar include:
- The meaning of hyperoxic, normoxic and hypoxic conditions at the cellular level
- How hyperoxic conditions affect the HIF1 transcription factor and its many downstream effects
- How oxygen is beginning to play a larger and larger role in cellular labs
- The future of hypoxic/normoxic cell culturing in research
Relevant publications & resources
- Al-Ani, A. et al (2018). Oxygenation in cell culture: Critical parameters for reproducibility are routinely not reported. PLoS ONE 13(10): e0204269.
- Wenger, R. et al (2015). Frequently asked questions in hypoxia research. Hypoxia 3: 35-43.
- Normoxia isn’t normal: Conventional incubators generate more questions than they answer
- Bringing hypoxia chambers into the 21st century
- HypoxyLab Brochure
To download a PDF copy of the presentation, click on the “LinkedIn SlideShare” icon located in the bottom-right corner of the slide-viewer. From the SlideShare landing page click the “Download” button to retrieve the file.
Nicky holds a Ph.D. from the University of Maastricht, in the Netherlands. His expertise includes skeletal muscle remodeling, cell culture and molecular biology. He has previously worked on cellular signaling pathways involved in muscle (cell) differentiation and (re)growth both in vitro and in vivo. Nicky is currently a product manager for Europe with Scintica Instrumentation.