Dr. Scott Earley and Dr. Gerry Herrera review essential tools and techniques for studying function in isolated blood vessels using a pressurized arteriograph system.
During this webinar sponsored by Living Systems Instrumentation, presenters review dissection and mounting techniques, equipment requirements, proper experimental design, and discuss the difference between pressurized cannulation systems and wire myograph/tissue bath systems.
Dr. Scott Earley shares strategies for skills development, in particular starting research with 2nd order mesenteric artery samples and how to generate vessel constrictions and dilations. In addition, he discusses an advanced application involving the assessment of myogenic reactivity in a “blind-sac” vessel preparation using a cerebral parenchymal arteriole.
Key topics in this webinar include…
- equipment needs and proper operation
- how to design an experiment to assess blood vessel function in-vitro
- techniques for vessel dissection, mounting, and assessing viability
- how to interpret experimental results
- studying myogenic responses using a cerebral parenchymal arteriole
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