An Introduction to Isolated Langendorff Heart: Experimental Considerations and Best Practices

An Introduction to Isolated Langendorff Heart: Experimental Considerations and Best Practices


Join Dr. Melanie White for a discussion on basic isolated Langendorff heart principles, key experimental design considerations, core technology requirements and best practice tips to support consistency and validation of your research.

The isolated Langendorff heart has been a cornerstone of cardiovascular research for more than a century. Its long-standing scientific relevance is partly due to the fact that it allows experimental conditions to be studied beyond confounding hormonal and neurological influence. In addition, the isolated perfused heart serves as a bridge between in vivo and in vitro research, with many applications in heart physiology and pharmacology.

In this webinar sponsored by ADInstruments, Dr. Melanie White, Heart Foundation Future Leader Fellow from the University of Sydney, provides a useful introduction to isolated heart studies.

Key topics covered during this webinar include:

  • Understanding the core principles of isolated Langendorff perfusion
  • Key methodological considerations for excision, cannulation and perfusion of the heart
  • Experimental design: when to use constant flow vs. constant perfusion, animal models (species, sex, age) and choice of anesthesia 
  • How to set up your hardware to ensure your experiments are trouble-free
  • Tips for data analysis: using a baseline period, defining exclusion criteria and evaluating functional output
  • Applications of Langendorff perfusion, from myocardial ischemia to diabetic cardiomyopathy

To download a PDF copy of the presentation, click on the “LinkedIn SlideShare” icon located in the bottom-right corner of the slide-viewer. From the SlideShare landing page click the “Download” button to retrieve the file.

Melanie White, PhD

Heart Foundation Future Leader Fellow
The University of Sydney School of Medicine

Dr. Melanie White is a member of the Charles Perkins Society, a Heart Foundation Future Leader Fellow and teaches senior students in the discipline of Pathology at the University of Sydney School of Medicine. Her research interests include understanding more about how cells adapt to their changing environment by altering proteins using post-translational modifications. Her and her team’s work centers on asking these questions in clinically relevant models of myocardial ischemia (heart attack), type 2 diabetes and obesity.