Online Neural Data Processing with Plexon OmniPlex: Referencing, Sorting and Online Manipulation

Online Neural Data Processing with Plexon OmniPlex: Referencing, Sorting and Online Manipulation

Learn the fundamentals of Plexon’s OmniPlex software, including how to improve signal quality, data recordability, and approach complex applications of spike sorting.

Scientists are faced with numerous challenges when planning and executing research involving high-resolution recordings of neural biopotentials in laboratory animals. Many of these challenges relate to the nuances of the hardware used to collect these measurements, while others touch upon signal acquisition and processing, data management, and downstream analysis.

To simplify the research processes, Plexon offers OmniPlex – a turn-key acquisition and data management platform for recording neural biopotentials from acute or chronically implanted electrodes in both anesthetized and awake, behaving animals. Understanding the core features available through this platform, and more importantly, when and how to apply them can help advance your research.

Therefore, the focus of this webinar is to showcase select features of the OmniPlex acquisition software essential to addressing common challenges experienced in the lab. Specifically, Andrew Klein, Director of Sales and Support at Plexon, demonstrates online digital referencing features, including Common Average Referencing (CAR) and Common Median Referencing (CMR), automatic thresholding, and manual and automatic sorting. In addition, viewers learn about features designed to assist researchers and solve common issues, such as recording in a noisy environment, difficulty sorting on many channels, and spike sorting poorly isolated data.

This is an essential webinar for scientists that wish to learn…

  • How to set-up online digital referencing with OmniPlex PlexControl software
  • The difference between hardware referencing at the point of acquisition and online digital referencing
  • The difference between manual thresholding and automatic thresholding, and why one is preferable over the other
  • Different methods for online spike sorting, focusing on manual verses automatic methods

Key topics covered during the presentation include:

  • CAR vs. CMR, and which one you should use
  • Online sorting – fundamental explanation of spike sorting vs. spike selection, differences between each method, automatic methods
  • Hardware referencing vs. digital referencing
  • Automatic thresholding & Trodal sorting

Introduction & Agenda: 0:01
Layouts: 3:49
Thresholding & Extraction – Manual Thresholding: 8:48
Automatic Thresholding: 15:47
Referencing (Hardware & Software): 20:29
Spike Sorting (Line Method): 28:41
Spike Sorting (2D Polygon): 34:30
Presentation Summary: 40:27
Q&A Session: 42:35

To download a PDF copy of the presentation, click on the “LinkedIn SlideShare” icon located in the bottom-right corner of the slide-viewer. From the SlideShare landing page click the “Download” button to retrieve the file.

Andrew Klein

Director of Sales and Support,
Plexon Inc.
Dallas, TX

Presenter Bio:

Andrew Klein is Director of Sales and Support at Plexon Inc. He obtained his BSc in Neuroscience from Bowling Green State University in 2005, and following spent time as a research associate at the University of Michigan before accepting a position at Plexon in 2008. He has over twelve years’ experience with in vivo electrophysiology data acquisition and animal behavior analysis. Today, he spends a significant amount of time traveling throughout North and South America, Europe, and Asia supporting users of Plexon electrophysiology acquisition systems.