Aaron Stupica and Dr. Keshari Thakali discuss techniques and best practices for tissue bath myography and present a unique case study looking at the effect of maternal obesity on offspring perivascular adipose tissue and vascular function.
Tissue organ baths are an invaluable tool for both clinical and preclinical physiology and pharmacology research. Part of their appeal is that perfused tissue can be tightly controlled and manipulated independent from the influence of the processes that occur in vivo, making data more consistent and reproducible.
In this webinar sponsored by DMT, Aaron Stupica and Keshari Thakali discuss fundamentals and unique preclinical applications of advanced tissue organ bath systems. Specifically, Aaron Stupica, Scientific Product Specialist at DMT provides an overview of operating procedure, reviews best practices and discusses how this technology can be used to study various smooth and striated muscle samples in vitro such as aortic rings, heart tissue and gastrointestinal tissue preparations.
Following, Dr. Keshari Thakali of the University of Arkansas for Medical Sciences presents her current research using organ bath systems to study the impact of maternal obesity on perivascular adipose tissue in offspring. She also discusses the role of adipose tissue surrounding arteries, how it can regulate arterial function, and how maternal obesity may program vascular function.
Key topics covered during this webinar include…
- Fundamentals of smooth and striated muscle research using tissue organ baths
- Experimental design and best practices
- How isolated tissue baths and myography can be used to study complex vascular disease and function
This webinar will be most valuable to novice researchers looking to better appreciate the fundamentals of tissue bath procedure and how to get started with this technology as well as experienced researchers who wish to learn more about advanced tissue bath systems and how they can benefit their current research.
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University of Arkansas for Medical Sciences
Dr. Keshari Thakali is an Assistant Professor at University of Arkansas for Medical Sciences and directs the Arkansas Children’s Nutrition Center Vascular Programming Lab. Her laboratory studies maternal nutrition, physical activity, and obesity, and how gestational exposure to maternal obesity can increase the risk of cardiovascular disease in offspring. Her lab continues to determine the physiological mechanisms giving rise to this risk, and how factors such as exercise and nutrition can affect the health and well-being of offspring. A primary area of interest of her lab is the study of perivascular adipose tissue (PVAT), a paracrine regulator of smooth muscle tissue, which, as some studies suggest, may be a key link between cardiovascular disease and obesity.
Scientific Product Specialist
Danish Myo Technology A/S (DMT)
Aaron Stupica has spent the last 6 years with Danish Myo Technology, leading DMT training courses and building relationships with customers to get the most out of their scientific instrumentation and provide input for product development in Denmark. Aaron earned a MS in Biological Engineering in 2011 and began his career at DMT the following year. Since that time, Aaron has gained significant experience in scientific sales and technical product support, being promoted to Scientific Product Specialist and Product Manager to better support the needs of North and South American customers, represent his work with the products, and connect researchers with the Danish development team to continually improve options available to the scientific community.