Turn Away from Traditional Tethering and Towards a Better Method for Data Collection

Turn Away from Traditional Tethering and Towards a Better Method for Data Collection

Experts discuss the use of a novel movement responsive rodent caging system as a means to minimize animal stress and enable unique discovery in many research applications, namely neuroscience, animal behaviour, drug discovery and cardiometabolic disease.

Novel environments (cage changes), traditional handling, restraint methods, and even the presence of humans can cause stress to laboratory animals. Minimizing stress to animals throughout the duration of a study is key to generating accurate, reproducible data while also improving animal welfare. BASi’s RaturnTM is a movement responsive caging system that facilitates data collection in awake, freely moving rodents without the need for human intervention! Unlike swivels, this allows for simultaneous collection of multiple types of data that can be obtained from study animals, making it a useful tool for any researcher conducting tether-based studies.

During this webinar sponsored by BASi, Candace Rohde-Johnson will discuss how tethering systems can be used as a hands-off approach to data collection for research applications including microdialysis, open flow microperfusion, telemetry and optogenetics.

Following, Dr. John Cirrito, leading Alzheimer’s disease researcher and professor at Washington University of St. Louis, will present an overview of the data his team has collected through an in vivo microdialysis technique that enables minute-to-minute measurement of interstitial fluid (ISF) Aβ within the brains of awake and behaving mice. He will also share additional study applications made possible by using the RaturnTM system.

Key topics covered during this webinar will include…

  • The impact of human presence on data collection in lab animals
  • How the RaturnTM can increase flexibility in study design and advanced applications
  • The relationship between synaptic transmission and ISF Aβ levels

Research Applications:

  • Addiction
  • Anesthesiology
  • Behavioral Neuroscience
  • Cardiovascular
  • Inflammatory Disease
  • Metabolic Disease
  • Neuroscience
  • Ocular Disease/Function
  • Oncology
  • Pain
  • Pharmacokinetics
  • Renal Physiology
  • Reproductive Toxicology
  • Safety Pharmacology
  • Sleep
  • Toxicology

Measurement Techniques:

  • Microdialysis
  • Open Flow Microperfusion
  • Optogenetics
  • Fiber Photometry
  • Neuronal Biopotentials (e-phys)
  • Blood Sampling
  • Microinfusion
  • Microinjection
  • EEG
  • EMG
  • Drug Infusion
  • Activity Monitoring
  • Commutators
  • Swivels
  • Telemetry

Click to watch the webinar recording. To view the presentation full screen simply click the square icon located in the bottom-right corner of the video-viewer.

To download a PDF copy of the presentation, click on the “LinkedIn SlideShare” icon located in the bottom-right corner of the slide-viewer. From the SlideShare landing page click the “Download” button to retrieve the file.


John Cirrito, PhD
Associate Professor of Neurology,
Washington University in St. Louis

Dr. Cirrito started his PhD in Neuroscience at Washington University in the lab of Dr. David Holtzman (Neurology) studying Alzheimer’s disease in 1999. Much of his PhD work focused on the proteins and cellular pathways that eliminate amyloid-beta (Aβ) from the brain. Here he developed the first use of in vivo microdialysis to study Aβ kinetics in the brain of a living mouse. This was the first time someone was able to measure Aβ peptide longitudinally. In the Holtzman lab he realized the importance and value of collaboration, having worked with numerous departments across the university as well as outside groups and industries. John received his PhD in 2005, then became a post-doc in the lab of Dr. Steven Mennerick (Psychiatry) where he learned to pretend to be an electrophysiologist to study synaptic processes that regulate Aβ generation. He started his own laboratory (Neurology) at WashU studying Alzheimer’s disease in 2010, received tenure in 2015.

Candace Rohde-Johnson
Director of in Vivo Products and Services,

Ms. Rohde-Johnson earned a B.S. in Anthropology/Zoology from the University of Michigan in 2003. She has worked in animal research for more than 14 years, beginning in the lab and moving into customer support and management roles. Throughout her career, Candace has worked with clients worldwide to provide training, seminars and workshops focused on improving research results without sacrificing animal welfare.