Marshall McCue, PhD presents case studies using small mammals where 13C-labeled nutrient oxidation is tracked second-by-second in real-time using the newest laser-based stable isotope approaches.
Stable isotope analysis is a powerful tool for obesity research. Nutrients labeled with these tracers are nontoxic to animal models and can be continuously tracked in vivo.
In this webinar, Dr. Marshall McCue will review stable isotope biochemistry, standard nomenclature, unit conversions and simple calculations. In addition, he will present case studies highlighting the use of novel laser-based stable isotople approaches to track 13C-labeled nutrient oxidation second-by-second in real-time. He will discuss how dietary (exogenous) and stored (endogenous) nutrients can be traced to identify several research questions, including how to tell if your animal is snacking on cached food and how experimental manipulations alter oxidation of stored lipids.
Key Topics Include:
Tracing the oxidative fates of dietary (exogenous) nutrients:
- How can I tell if my animal is snacking on cached food?
- When does my animal begin to oxidize the food it eats?
- How do experimental manipulations alter oxidative kinetics of dietary nutrients?
- Does my animal preferentially oxidize some types of dietary fatty acids over others?
- How are different dietary sugars and amino acids differentially used by my animal?
Tracing the oxidative fates of stored (endogenous) nutrients:
- What are the limitations for interpreting respiratory exchange ratios?
- How can I selectively enrich specific nutrient pools in the body of my animal?
- How do experimental manipulations alter oxidation of stored lipids?
- How does exercise and activity affect lipid and protein oxidation?
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Sable Systems International
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