VO2 MAX Test Optimization for Cardiometabolic Phenotyping in Rodents

VO2 MAX Test Optimization for Cardiometabolic Phenotyping in Rodents

Attend this webinar to learn how VO2 Max exercise testing can be used to assess the cardiometabolic phenotype of rodents.

Coronary Heart Disease is the number one killer in the world, and while a great number of tests exist, they don’t always downsize gracefully for mouse models. The Graded Exercise Test (GXT), the gold standard in assessing cardiopulmonary function, is a perfect example of a test that is far more challenging to reproduce in mice. While getting mice to run a treadmill is rather straight forward, accurately and consistently assessing VO2 MAX is not. Measured VO2 peaks that fall short of VO2 MAX or have endless plateaus are common frustrations.

Obtaining quality data means balancing a number of compromises. These compromises span the entire experiment from instrumentation, protocol, and calculations. During this webinar, sponsored by Columbus Instruments, Jennifer Petrosino will discuss her research in the mechanisms that regulate muscle growth and repair and aim to empower researchers with a stable base by pointing out key instrumentation features, an easy to follow protocol, and equations for cardiometabolic phenotyping.

Key Learning Objectives Include:

  • Understanding oxymax output measurements and derived calculations from output data during exercise tests
  • Optimization of Indirect Calorimetry setup for VO2 Max testing
  • Significance of VO2 Max, the graded max exercise test and the criteria for a good VO2 Max test
  • How exercise testing endpoints can be used to assess mouse phenotype
  • Additional calculations for phenotyping (crossover, fuel utilization, Tachycardia)

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Presenters:

Jennifer Petrosino
PhD Candidate
Ohio State University

Jennifer Petrosino is a Ph.D. candidate in the Biomedical Sciences program at Ohio State, a National Institute of Arthritis and Musculoskeletal and Skin Diseases F31 Fellow, and a member of the Accornero Lab. Her work focuses on understanding the molecular mechanisms regulating how muscles grow and repair and how fat accumulates and remodels. Prior to that she received an M.S. in Kinesiology from Ohio State, where she worked on developing translational exercise tests in mice, using indirect calorimetry treadmills, to assess their cardiometabolic phenotypes. Her B.S. is in exercise science from the University of Miami (FL), where she worked with the University’s strength and conditioning department. She is originally from Los Angeles, California.

Chris Adams
Sales Manager and Marketing Director
Columbus Instruments

Sales Manager and Marketing Director at Columbus Instruments in Columbus, Ohio. Chris has 20 years of experience helping customers configure robust and reliable solutions for a variety of research applications encompassing metabolism, exercise, and neuroscience.