An exclusive training workshop for cardiovascular researchers interested in non-invasive doppler flow velocity measurements as a means to study vascular biology, hemodynamics and cardiac function in small rodents.
During this course, scientists will learn experimental strategy and procedure for successful non-invasive cardiac research using established Doppler Flow Velocity measurement techniques. The course includes review of important fundamental concepts including proper sedation, assessment of electrocardiogram, temperature control and respiration.
In addition to these fundamentals, attendees will learn how to measure blood flow velocity from various sites in the cardiac and systemic circulatory system, including the coronary left main artery, using non-invasive Doppler Flow Velocity measurements, all while simultaneously tracking vital physiological signals including heart rate, respiration rate and core body temperature. Specifically, attendees will learn how to measure and analyze baseline and post-intervention blood flow velocities to determine indices of systolic function (peak aortic flow velocity), LV contractility (aortic acceleration), diastolic function (E/A ratio), LV lusitropy (DT), coronary flow reserve (baseline/hyperemic coronary flow) and aortic stiffness (pulse wave velocity).
Utilizing these functional measurements is essential for the effective study of various cardiovascular and hemodynamic disease models, including hypertension, atherosclerosis, arterial stiffness, cardiac hypertrophy and heart failure models, to name a few. In addition to learning best-practices in non-invasive monitoring and measurement techniques, attendees will also have an opportunity to discuss experimental design and research objectives with course instructors.
Doppler Flow Velocity and Rodent Surgical (vital sign) Monitoring equipment for this course has been graciously supplied by Indus Instruments. Learn more about these technologies by visiting www.scintica.com, their global product distribution and support partner.
Baylor College of Medicine
Industry: $3,295 (USD)
Academic: $2,795 (USD)
- Fundamentals of physiological and ultrasound measurements in preclinical cardiovascular disease
- Key physiological principles – signals, sampling and noise issues
- Key ultrasound principles – pulsed Doppler concept- range/depth/angle, signals, sampling/processing, aliasing, frequency
- Understanding the differences between blood flow & blood flow velocity
- Cardiovascular anatomy: cardiac sites & vascular sites
- Cardiac function: measuring blood flow velocity at aortic root, at mitral orifice in rodents (mice) to determine systolic & diastolic function, respectively
- Coronary function: measuring blood flow velocity in left main coronary artery in rodents (mice) to determine coronary flow reserve
- Vascular function: measuring blood flow velocity at aortic arch and abdominal aorta in rodents (mice) to determine pulse wave velocity to estimate aortic/arterial stiffness
- Measurement procedures, signal qualification, data analysis and interpretation
Topics of Discussion:
- How could specific cardiovascular diseases be modeled in this animal or subject group?
- What can we measure to diagnose and study certain cardiovascular diseases?
- How does the cardiovascular system adapt and compensate with disease?
- How these physiological compensations effect the model, measurements and interpretation of results?
Dr. Anil K. Reddy, PhD
Dr. Reddy’s research interests include evaluation of cardiac and vascular mechanics in senescent, disease, transgenic, and surgical models of mice. Some of the rodent models he studies include atherosclerosis, dwarf, myocardial infarction/remodeling, pressure overload, hypertension, absent vascular tone, and absent steroid receptor coactivator-1, with the main goal being to translate what is learned in mice to humans for early detection and screening. Using noninvasive methods, such as pulsed Doppler Flow Velocity measurements as well as imaging methods, animals are phenotyped as abnormalities develop and progress, and their cardiovascular system is monitored as it adapts and compensates for the deterioration of function or for missing or over-expressed proteins. The main goal is to translate what is learned in mice to humans for detection and screening of cardiovascular diseases at an early stage when potential therapies can be most effective at preventing disease progression.