Rodent Microsurgery and Hemodynamic Measurements Training Program
-- Resources --
As a workshop registrant you are permitted access to the following program resources. Some of the content provided in this area is intended for workshop registrants only — please do not share or distribute these articles, unless otherwise noted.
Our recommendation is to review all course material before visiting the laboratory in Madison, Wisconsin for hands-on training. Having an appreciation for the various hardware, software, and surgical instrumentation that will be used during the course will improve your educational experience in the lab.
If you have any questions in advance for Dr. Hacker, or wish to seek further details about the instrumentation that will be used in the lab, please send your requests to [email protected]. Course administrators will be happy to provide assistance wherever necessary.
Basic Hemodynamic Principles Viewed Through Pressure-Volume Relations
This is an overview of the fundamental principles of cardiac muscle physiology and hemodynamics, including the concepts of preload, afterload, contractility and lusitropy (diastolic properties). Dr. Burkhoff reviews how these are quantified on the pressure-volume diagram and how they are affected in heart failure. Furthermore he demonstrates how pressure-volume analysis can lead to a quantitative understanding of heart-vasculature interaction, and how to determine stroke volume, cardiac output and blood pressure.
Advanced Concepts in Pressure-Volume Analysis
In this session, Dr. Burkhoff discusses advanced concepts of PV analysis, including best-practices during data acquisition and statistical analysis. There is a focus on load-independent measurements, including possible ESPVR changes in response to intervention and how to linearize EDPVR data to do analysis of covariance. An extended Q&A session was included which focused on the following: the importance of Tau, Arterio-Ventricular Coupling, changes to PV Loops in Valve Lesions, RV vs. LV Pressure-Volume Loops, and more.
General Surgery Training — Essential Concepts and Tools
Tracy Fontaine, Technical Services Manager for the Americas at Harvard Apparatus, provides an overview of essential laboratory equipment required for successful rodent microsurgery. The following concepts are discussed: Surgical Preparation, Surgical Instruments, Anesthesia, Ventilation, and Post Operative Recovery. An introduction to the surgical tools, ventilators, and anesthesia vaporizers that attendees will use in the lab is also included.
An Introduction to Surgical Monitoring
During this session the value and need for integrated surgical monitoring in rodent research applications is discussed. Key concepts include… how integrated surgical monitoring improves surgical outcomes and overall research endeavors, NRC & IACUC Guidelines, Documentation requirements, and how to properly monitor ECG, Respiration Rate, Body Temperature, and Blood Gases. An introduction to the Rodent Surgical Monitor (MouseMonitorS) that attendees will use in the lab is also included.
Data Acquisition Essentials for Cardiovascular Research Applications
Brandon Bucher of ADInstruments introduces LabChart software and PowerLab data acquisition hardware with a focus on recording and analyzing cardiovascular measurements including blood pressure, blood flow, and PV Loops. The following concepts are discussed: setting up acquisition parameters such as sampling frequency and range, input signal calibration, creating template files, basic waveform analysis for pressure and flow, and recording Admittance PV Loops and utilizing basic functions for hemodynamic analysis.
Advanced Data Acquisition for Hemodynamics
In this second installment, Brandon Bucher covers select advanced data analysis techniques using LabChart software for studying blood pressure, blood flow, and PV Loops. Key functions include… using Analysis modules for blood pressure and PV Loops, channel calculations & data pad tools for blood flow including peak analysis, and using LabChart Analysis Manager.
Introduction to Pressure, Flow, and Pressure-Volume Loop Measurements
Cole McLarty, Research Sales Specialist and National Trainer at Transonic, provides an introduction to measuring systemic blood pressure, blood flow, and ventricular pressure-volume loops. The following concepts are discussed: the technologies associated with solid-state pressure catheters and transit-time flow probes, an introduction to the PV Loop and its value in studying hemodynamics and cardiac dysfunction, and the “Admittance” method of volume derivation using a PV catheter. A review of Transonic hardware, catheters, and flow-probes that attendees will use in the lab is also included.
Advanced Applications of Hemodynamics in Preclinical Research
Filip Konecny, Application Scientist & Surgical Trainer at Transonic, discusses the value of advanced pressure-flow and measurements including Pulse-Wave Velocity (PWV), Pulmonary Artery Pressure (PAP) and PAP Augmentation Index. In addition, he reviews important concepts of Admittance Volume derivation, including interpretation of Magnitude and Phase signals collected from the PV catheter, how to ensure these signals are recorded accurately and consistently, and executing a proper “baseline scan” to ensure resulting real-time blood volume is calculated properly. Good and bad data examples are included to help scientists better understand how to work with the PV system at the bench.
Low-Flow Isoflurane Anesthesia for Mice and Rats
In this short video, Kim Matheny, Product Support Specialist at Kent Scientific, provides an overview of inhalant vs. injectable anesthesia, the limitations of traditional high-flow vaporizers, and the low-flow vaporizer alternative. She discusses isoflurane use, the inherent dangers of waste anesthetic gas, and how these dangers can be mitigated by using a low-flow system. Kim ends the session by describing each of the add on modules for warming, ventilation, and additional vital sign monitoring systems.
The following section includes additional resources for workshop attendees. You will find application notes, technical product guides, workbooks, and a variety of other resources all related to studying hemodynamics in rodents. Resources are sorted by Source.