Experts discuss the utility of SEC-MALS in characterizing protein oligomerization and how it can complement protein structure determination and prediction tools.

Newly available genomic data on diverse organisms, coupled with the development of new structural tools like cryo-electron microscopy and powerful structure prediction algorithms, has opened new frontiers in exploring novel biological pathways. A key step in understanding many new pathways is the identification and characterization of functional protein-protein complexes. This webinar summarizes the utility of SEC-MALS in characterizing oligomeric states of protein homo- and hetero-complexes, and show how these data complement different structural methods to drive fundamental discoveries. To illustrate each concept, the webinar will present case studies on bacteria-bacteriophage conflict.

Key Topics Include:

  • Understand the basic principles of SEC-MALS and its application to purified proteins in aqueous solution
  • Explore the use of fusion tags and protein engineering to understand protein oligomerization
  • Learn how SEC-MALS can complement structure determination tools like X-ray crystallography and cryo-electron microscopy
  • Learn how SEC-MALS can complement structure prediction tools like AlphaFold and RosettaFold

Who Should Attend?

  • Protein biochemists
  • Structural Biologists who want to understand protein behavior
  • Managers of academic labs and protein production/characterization core facilities who want to broaden the analytical methodology


Application Scientist in Marketing
Wyatt Technology

Nayanthara Dharmaratne is the Marketing Application Scientist at Wyatt Technology, responsible for creating and producing technical marketing content. Nayanthara received her Ph.D. in Physical Organic Chemistry, with a focus on polymer synthesis, from the University of Rhode Island, and subsequently carried out postdoctoral work at the Yale University School of Medicine, where she studied and developed gene delivery systems for cancer-targeted therapy.

Dept. of Cellular and Molecular Medicine
University of California, San Diego

Kevin Corbett received his Bachelor’s degree in Biochemistry and Biology from the University of Virginia, and his PhD in Molecular Cell Biology from UC Berkeley. At UC Berkeley, he studied the structure and function of DNA topoisomerases from bacteria and archaea. As a postdoctoral fellow at Harvard Medical School, he studied the structural basis for kinetochore-microtubule attachments in eukaryotic mitosis and meiosis. Since 2011, Dr. Corbett has been a professor in the Department of Cellular and Molecular Medicine at UC San Diego. His lab uses biochemistry and structural biology to understand the molecular mechanisms of genome maintenance in diverse contexts, including eukaryotic meiosis and bacteria-phage conflict. He is well versed with light scattering techniques and uses multi-angle light scattering technology as a tool for biophysical characterization of proteins.

Production Partner

Wyatt Technology

Wyatt Technology is the recognized leader in light scattering instrumentation and field-flow fractionation systems for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution. Wyatt’s scientific tools support the pharmaceutical industry, academia and government labs worldwide, in the research and development of novel therapeutics including vaccines, gene vectors, nano-drug carriers, monoclonal antibodies, and other advanced pharmaceuticals, as well as fundamental life and material sciences.

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