Experts discuss the utility of SEC-MALS in characterizing protein oligomerization and how it can complement protein structure determination and prediction tools.
Newly available genomic data on diverse organisms, coupled with the development of new structural tools like cryo-electron microscopy and powerful structure prediction algorithms, has opened new frontiers in exploring novel biological pathways. A key step in understanding many new pathways is the identification and characterization of functional protein-protein complexes. This webinar summarizes the utility of SEC-MALS in characterizing oligomeric states of protein homo- and hetero-complexes, and show how these data complement different structural methods to drive fundamental discoveries. To illustrate each concept, the webinar will present case studies on bacteria-bacteriophage conflict.
Key Topics Include:
- Understand the basic principles of SEC-MALS and its application to purified proteins in aqueous solution
- Explore the use of fusion tags and protein engineering to understand protein oligomerization
- Learn how SEC-MALS can complement structure determination tools like X-ray crystallography and cryo-electron microscopy
- Learn how SEC-MALS can complement structure prediction tools like AlphaFold and RosettaFold
Who Should Attend?
- Protein biochemists
- Structural Biologists who want to understand protein behavior
- Managers of academic labs and protein production/characterization core facilities who want to broaden the analytical methodology
Application Scientist in Marketing
Dept. of Cellular and Molecular Medicine
University of California, San Diego