Metabolic researchers need simultaneous multi-parameter measurements of Respiratory Exchange Ratio (RER), animal activity and food intake to properly asses metabolism. However, selecting the correct equipment and aligning data can be challenging. This webinar discusses the essential elements of metabolic research and how they are to be applied.

In this exclusive event sponsored by Columbus Instruments, Chris Adams provides an overview of essential parameters for studying energy expenditure in laboratory rodents. He reviews gas exchange using open circuit indirect calorimetry to measure VO2, VCO2, RER and energy expenditure; food/water intake using direct mass measurement for accumulative and/or bout monitoring for controlling caloric intake and for identifying diet preference; and activity using an IR photo-cell array and/or running wheel rotation for scoring locomotion, stereotypic behavior (grooming/scratching), resting behavior and wheel running behavior.

In addition, Dr. Marta Fiorotto presents quantitative data acquired in her lab such as energy intake and energy expenditure while highlighting qualitative measurements such as spontaneous cage activity, voluntary activity and dietary-induced thermogenesis. She discusses the value of “secondary data” as well with a focus on RER and behaviors such as eating patterns, sleeping and exploratory drive. Finally, Dr. Fiorotto highlights key considerations for proper experimental design and to ensure data validity including habituation, animal-to-animal individuality, diets, environmental factors, instrument setup, timing of measurement and normalization protocols.

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Presenters

Associate Professor, Pediatrics-Nutrition
Baylor College of Medicine

Sales Manager
Columbus Instruments

Production Partner

Columbus Instruments

Columbus Instruments manufactures products for animal activity, energy expenditure, feeding and drinking monitors, rodent exercise treadmills, rota-rod, grip strength, running wheels, analgesia hotplate and tail-flick, PPI startle response, end-tidal CO2 monitors, non-invasive tail cuff blood pressure, and their flagship CLAMS and CLAMS-HC metabolic phenotyping systems.

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