Specialists discuss a novel radiopaque polymerizing contrast agent called µAngiofil that can be used to overcome the inherent challenges in distinguishing vasculature from soft tissues using micro CT.

In recent years, non-destructive 3D imaging techniques such as micro-computed tomography (microCT) have increasingly captured the attention of preclinical researchers across almost every discipline. For angiography, microCT provides volumetric data at high resolution, but the technique’s inherent X-ray attenuation means that vasculature cannot be distinguished from surrounding soft tissues.

In this Bruker BioSpin webinar, Dr. Ruslan Hlushchuk, from the Universität Bern Institute of Anatomy, will introduce a novel radiopaque polymerizing contrast agent called µAngiofil that can be used to overcome this challenge. Specifically for ex vivo angiography, µAngiofil enables resolution down to the fine-capillary level. In addition, samples can be used for subsequent morphological and quantitative analysis without extensive post-processing. Then he will describe the microCT-based visualization that can be achieved with µAngiofil and the perfusion procedure, visual controls and sample harvesting and handling involved. Bruker Biospin’s Skyscanner 1272 will be introduced, as well as the higher energy Skyscanners 1273 and 2214 that enable the imaging of denser samples.

This webinar will be of particular interest to facility managers, contract research organizations and preclinical researchers who specialize in cardiovascular-, neurovascular-, implantology- and angiogenesis-related fields.

Key Topics Include:

  • The perfusion procedure/protocol for µAngiofil – visual control of the perfusion quality, sample harvesting and tendering.
  • Non-destructive imaging of microvasculature down to the finest capillaries, as well as in the presence of bone tissue and/or metal implants.
  • The potential for correlative morphology
  • Examples of quantitative analysis

Presenters

Lecturer and Group Leader
Topographic and Clinical Anatomy, Institute of Anatomy
Universität Bern

Dr. Ruslan Hlushchuk, Lecturer and Group Leader of Topographic and Clinical Anatomy at the Universität Bern Institute of Anatomy. Head of the micro-CT group, Ruslan specializes in (micro)vascular imaging and microangioCT.

Production Partner

Bruker BioSpin

Bruker offers preclinical imaging solutions for a broad spectrum of application fields, such as cancer research, neuroimaging and cardiac disease.

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