High Throughput Investigation of EC Coupling in Isolated Cardiac Myocytes

High Throughput Investigation of EC Coupling in Isolated Cardiac Myocytes

Michiel Helmes, PhD presents new technology and methodology for fast data acquisition and functional analysis of cardiac myocytes, leading to better lab practices and data interpretation.

Measuring and analyzing calcium and contractility in isolated cardiomyocytes offers important insights into cardiac function. However, traditional methods of obtaining EC coupling data are somewhat limited to lower throughput — for many applications, particularly drug discovery research, this presents a big challenge. Additionally, low throughput data acquisition and analysis may lack the statistical power necessary to fully resolve differences, or changes, in cardiac function. Isolated myocytes can behave heterogeneously, thus greater sample numbers are essential for accurate and reliable modeling of cardiac behavior.

During this webinar sponsored by IonOptix, Michiel Helmes, PhD discusses recent advancements in instrumentation that address the shortfalls of low throughput EC coupling characterization. Specifically, Dr. Helmes explains the technology behind faster data acquisition and analysis, as well as improvements to the studies that offer more data acquisition fidelity, and automated data collection. He offers insights into best-practices for proper EC coupling measurement and highlight improvements to data handling, namely faster, automated data analysis.

Key topics covered during this webinar will include…

  • a new approach (and tool) for faster, more reproducible data acquisition
  • best practices for more reliable data collection
  • automated batch analysis — learn how to quickly and accurately analyze data from multiple myocytes at once
  • data interpretation — understand what your data means as it relates to cardiac function

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Michiel Helmes, PhD
Department of Physiology
VU University Medical Center Amsterdam
& IonOptix

Dr. Michiel Helmes has been able to successfully combine academia with an industrial career. After his PhD studying titin mechanics in cardiac muscle with Dr. Granzier, he has kept various academic appointments, at Boston University, the University of Oxford, and now the VU University Medical center in Amsterdam, The Netherlands. He is part owner and involved in system development at Ionoptix LLC. Being so fully immersed in working with isolated cardiac myocytes, he reached the conclusion that the field needed a more standardized instrument, and in 2015 he set up Cytocypher, that developed the high throughput system for calcium/contractility measurements that is the subject of this webinar.