Utilizing Noninvasive Blood Flow Velocity Measurements for Cardiovascular Phenotyping in Small Animals

Utilizing Noninvasive Blood Flow Velocity Measurements for Cardiovascular Phenotyping in Small Animals

A webinar for cardiovascular researchers interested in using noninvasive blood flow velocity measurements to quantify changes in hemodynamics and characterize cardiac disease without the need for complex surgery or imaging.

A variety of parameters exist for studying cardiac function, and each has its value in the complement of indices used to characterize and assess performance as part of a preclinical cardiac research protocol. Arguably, one of the most important parameters is the measurement of blood flow which can be monitored and acquired in different ways. Doppler flow velocity measurement is one technique that has been proven over the last 20 years in its ability to translate results from bench to bedside. Flow velocity measurement is noninvasive, obviating the need for surgical implantation of transducers, and offers researchers a technology option with unparalleled reduction in measurement error. This webinar, sponsored by Indus Instruments, shows why blood flow velocity measurement should be considered as an essential component for any study protocol in a cardiac research lab.

Dr. Anilkumar Reddy of the Baylor College of Medicine presents data from his research outlining the importance of blood flow velocity measurement and shows examples of translational data. He provides a brief overview of Doppler flow velocity measurement technology and compares data obtained from complimentary devices such as 3D echo ultrasound and transit-time flow systems. Several models are presented showing how many selected measurements scale up in translational research from mice to mammals.

During this webinar viewers learn how Flow Velocity measurements can reliably assess the following parameters in rodents:

  • Systolic and diastolic cardiac function
  • Myocardial perfusion & coronary reserve
  • Pressure overload
  • Aortic stiffness
  • Peripheral perfusion

Click to watch the webinar recording. To view the presentation full screen simply click the square icon located in the bottom-right corner of the video-viewer.

00:00 – 01:41:  Introduction

01:42 – 32:55:  Part 1

  • Pulsed Doppler Ultrasound – How does it work and How is it used
  • Technology overview and comparisons
  • Measurements that can be made and their anatomical sites
  • Calculating Pulse Wave Velocity (PWV) in mice

32:57 – 44:15:  Part 2

  • Transaortic Constriction (TAC) in mice
  • Pulsatility and Resistivity indicies to understand response of vasculature
  • Measuring Coronary blood flow in mice
  • Summary of Pulsed Doppler Capabilities and Applications

44:35 – 59:07: Q&A Session

To download a PDF copy of the presentation, click on the “LinkedIn SlideShare” icon located in the bottom-right corner of the slide-viewer. From the SlideShare landing page click the “Download” button to retrieve the file.

Dr. Anilkumar K. Reddy

Assistant Professor,
Medicine-Cardiovascular Sciences
Baylor College of Medicine

Presenter Bio:

Dr. Reddy’s current research interests include evaluation of cardiac and vascular mechanics in senescent, disease, transgenic, surgical models of mice. Some of the mouse models he studies include atherosclerosis, dwarf, myocardial infarction/remodeling, pressure overload, hypertension, and absent vascular tone. Using noninvasive methods in his lab, he phenotypes animals as abnormalities develop and progress, and monitors the cardiovascular system as it adapts and compensates for deterioration of function or for missing or over-expressed proteins. The main goal in his laboratory is to translate what is learned in mice to humans for early detection and screening of vascular diseases at an early stage when potential therapies can be most effective at preventing disease progression.