A presentation on key issues effecting the accuracy and reproducibility of measuring Oxygen Consumption, VO2, via indirect calorimetry utilizing Metabolic Carts, and best-practices for scientists to drive more consistent and precise measurements.
Metabolic Carts have been used for decades as a means of measuring Oxygen Consumption, VO2, via indirect calorimetry. However, the accuracy of these systems is generally not well known nor well understood — there is no universally accepted standard for determining the accuracy, or the validity, of Metabolic Cart measurements. In addition, scientists often do not have access or an appreciation for the specific calculations that are being used by these systems to calculate VO2 and other metabolic data. Collectively, this makes it challenging for researchers and physicians to understand if their data is valid.
In this webinar, sponsored by AEI Technologies, Phil Loeb introduces the measurements, variables and factors in determining VO2. He examines common sources of error and challenges that influence accuracy and reproducibility, and reviews validation methods commonly used by the research community. Following, Danny Rutar, Managing Director of Redback Biotek, presents best-practices, as embodied by the MOXUS Metabolic Cart, that are proven to minimize data error.
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