Visualizing the complex spatiotemporal dynamics of human stem cells as they proliferate and make cell fate decisions is key to improve our understanding of how to robustly engineer differentiated tissues for therapeutic applications.

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In this webinar, Dr. Rafael Carazo Salas describes multicolor, multiday high-content microscopy pipelines that his group has recently developed to visualize the dynamical cell fate changes of human Pluripotent Stem Cells (hPSCs). In particular, he reviews the integrated experimental and computational approaches that his group has established, including novel “live” reporters of cell fate and multi-reporter hPSC lines generated by CRISPR/Cas9 allowing multiplexed monitoring of cell proliferation and fate dynamics, and exemplify the biological discoveries they are enabling.

Key Topics Include:

  • Visualizing how human Pluripotent Stem Cells (hPSCs) proliferate and undergo early differentiation in vitro, by high content microscopy
  • Learning about experimental and computational pipelines that enable cell fate monitoring at the collective and single-cell level
  • Learning about novel “live” reporters of hPSC cell fate

Resources

#ExpertAnswers: Rafael Carazo Salas on Multicolor High Content Microscopy

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Presenters

Rafael E Carazo Salas, ;PhD

Rafael E Carazo Salas, PhD

Professor
School of Cellular and Molecular Medicine
University of Bristol

Dr. Rafael Carazo Salas's group seeks to better understanding the molecular networks and processes that control cellular growth, division and differentiation. Recently, they have actively pioneered technologies to study how pluripotency and differentiation are controlled using human embryonic stem cells and induced pluripotent stem cells. Their research goal is to understand how to specifically, efficiently and safely program heterogeneous populations of stem cells for therapeutic applications.

Production Partner

Yokogawa

Yokogawa's microscopy and life sciences solutions are designed to support applications from basic research to drug discovery and pre-clinical trials. Their high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, pharmaceutical research, and precision medicine to deliver rapid, high-resolution live cell imaging.

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