A Breath of Not-So-Fresh Air: the Combined Effects of Air and Noise Pollution
While air and noise pollution have been well documented independently, their combined effects are insufficiently discussed, until recently. In this blog post, we discuss a recent publication from Kuntic et al. on the combined and individual effects of these pollutants in mice.
Leveraging Programmable CRISPR-Associated Transposases for Next-Generation Genome Engineering
Dr. Sam Sternberg discusses a novel CRISPR-Cas9 system using programmable, RNA-guided transposase, and highlights its implications for kilobase-scale genome engineering in cell and gene therapies.
Synthetic Peptide Growth Factors: Forging the Path Forward for Regenerative Medicine, Cell Therapy, and Cellular Agriculture
Experts discuss how synthetic growth factors work and share application examples and data using these synthetic peptides for cell therapy and regenerative medicine.
Cancer Immunotherapy: Viruses, Vaccines, and other Immuno-Oncology Treatments
In this blog post, current cancer immunotherapy treatments are discussed, as well as some future directions for this rapidly developing field.
Celebrating Black Scientists: 5 Scientific Stories from the Past
In honor of Black History Month, this blog discusses 5 scientific stories from some historic Black and African-American scientists, researchers, and medical professionals.
Industry Insights with Crown Bioscience: Analyzing the Suppressive TME in In Vitro Based AssaysDarius Soo Lum
Nataliia Beztsinna and Marten Hornsveld from Crown Bioscience talk about native and reconstituted TME assays, how they can help in the preparation for clinical trials, and what kinds of therapies in development would benefit the most from this kind of testing.
Industry Insights with Crown Bioscience: Analyzing the Suppressive TME with In Vitro Based Assays
This episode of Industry Insights features Nataliia Beztsinna, PhD, and Marten Hornsveld, PhD, from Crown Bioscience as they describe how reconstituted TME and patient-derived ex vivo tissue assays can best be used within the drug development pipeline.
Protocol Preview: Long-read sequencing, Nature’s Method of the Year
Nature Methods declared "Long-Read Sequencing" their Method of the Year for 2022, but what exactly makes this technique worthy of such a title?
The Blues & the Browns: Links between Depression and Gut Microbiota
Recently, Radjabzadeh et al., identified 13 genera of gut microbiota that have been implicated with symptoms of depression.
Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal
Join Pilhan Kim, PhD for an in-depth discussion on the All-in-One intravital two-photon and confocal microscopy system.
The 3D DILI Model of Success – from Biological Relevance to Industry Application
Dr. Anna Borgström and Dr. Frauke Greve present the latest advancements in hepatic safety assessment using 3D liver spheroids.
#ExpertAnswers: Audrey Dubourg and Gareth Guenigault on the Organ-On-A-Chip ApproachDarius Soo Lum
Audrey Dubourg and Gareth Guenigault from CN Bio discuss the value that an organ-on-a-chip approach can bring to drug discovery and development workflows, and how the regulatory landscape is shifting to encourage their widespread adoption.
Protocol Preview: In Vivo Gene Therapy Using Peptide-Based Delivery
Although gene therapy has been in discussion for decades, its translation to clinical practice has been a slow and challenging process. As a solution to this translational challenge, Allen et al. have proposed a peptide-based delivery method of Cre recombinase for in vivo gene therapy, which we review in this blog post.
Industry Insights with Crown Bioscience: Targeting T Cells Within the Cancer Immunity CycleDarius Soo Lum
Saskia De Man, Marrit Putker, and Pirouz Daftarian from Crown Bioscience discuss the impact of T cell-directed immunotherapy on the cancer-immunity cycle.
#ExpertAnswers: Marrit Putker, Saskia De Man, and Pirouz Daftarian on T Cell-Directed ImmunotherapyDarius Soo Lum
Marrit Putker, Saskia De Man, and Pirouz Daftarian from Crown Bioscience discuss T cell-directed immunotherapy on the cancer-immunity cycle, including mechanisms and targets capable of modulating and enhancing T cell activity and the application of methods and models including organoids and high content imaging.
Novel Targets for Alzheimer’s Disease Treatment: Medin and PLD3
Given that Alzheimer's disease is multifactorial and heterogenous, drug development progress is more likely to occur if multiple pathways are considered. Two recent Nature publications have potentially identified medin and PLD3 as new targets for Alzheimer’s disease treatment, which we summarize in this mini-review.
#ExpertAnswers: Dudley Lamming on the Influence of Sex and Genetic Background on DietDarius Soo Lum
Dudley Lamming, PhD, explains that “We Are More Than What We Eat”; Sex and Genetic background are important factors in observing responses to diet in mice.
#ExpertAnswers: Rajendra Kumari and Gera Goverse on the Cancer Immunity CycleDarius Soo Lum
Rajendra Kumari and Gera Goverse provide an overview of the cancer immunity cycle and present several methods, techniques, and model systems for immuno-oncology research.
#ExpertAnswers: Oscar Perez Leal and Jon Weidanz on Gene EditingDarius Soo Lum
Oscar Perez Leal and Jon Weidanz discuss the basis of the FAST-HDR gene-editing system to create custom cell lines, and present case studies highlighting several applications.
#ExpertAnswers: Amy Sheng on Antibody Screening and DiscoveryDarius Soo Lum
Amy Sheng provides an overview of antibody screening platforms and presents applications and case studies using the Beacon® platform for antibody discovery.
#ExpertAnswers: Ejvind Mørtz on Protein AnalysisDarius Soo Lum
Ejvind Mørtz discusses the benefits and applications of mass spectrometry (MS)-based host cell protein (HCP) analysis and why HCP ELISAs are insufficient for documenting product-related impurities.
#ExpertAnswers: Jonathan Brestoff on Energy ExpenditureDarius Soo Lum
Jonathan Brestoff explains how energy expenditure can be better described thanks to a new bioinformatic algorithm known as Clambake.
#ExpertAnswers: Michael Sturek on Macrovascular Atherosclerosis and Microvascular DysfunctionDarius Soo Lum
Michael Sturek reviews features of macrovascular atherosclerosis and microvascular dysfunction that underlie ischemic events and the need for appropriate animal models for optimal translation.
#ExpertAnswers: David Barefield on Atrial DysfunctionDarius Soo Lum
David Barefield discusses his work investigating the role of the Myosin binding protein H-like (MyBP-HL) protein in atrial dysfunction.
#ExpertAnswers: Eduardo Marbán on Regenerative Cardiovascular MedicineDarius Soo Lum
Eduardo Marbán dives into the complexities of regenerative cardiovascular medicine and the future directions for cell therapies.