Techniques and Best Practices for Cardiomyocyte Isolation

Techniques and Best Practices for Cardiomyocyte Isolation

Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies, including key considerations, best practices and how to achieve and confirm optimal myocyte quality.

For the successful study of cardiomyocytes, proper isolation and assessment of myocyte viability is imperative. If a researcher’s isolation technique is not fundamentally sound, data quality will be compromised, confounding any results and conclusions. Therefore, having a clear understanding of these techniques is essential for any scientist using myocytes in their research.

In this webinar sponsored by IonOptix, Dr. Ronglih Liao of the Stanford School of Medicine and Dr. Davor Pavlovic of the Institute of Cardiovascular Sciences at the University of Birmingham address these long-standing hurdles by discussing best practices for isolating healthy, calcium-tolerant adult cardiomyocytes for functional studies. They describe their techniques, the basic principles and theory behind these methodologies and modifications that should be considered based on an experiment’s species and reagents.

Specifically, Dr. Liao presents a proven, Langendorff-based method and discusses how to evaluate the success of an isolation based on calcium tolerance, morphology and functional outputs. Following, Dr. Pavlovic reviews an innovative Langendorff-free method for cardiomyocyte isolation, compares and contrasts this approach with the traditional procedure and discusses the pros and cons of each.  The webinar closes with an engaging Q&A session with the presentation team.

This is an essential webinar for basic researchers utilizing isolated cardiac myocytes, those who have considered using them but have found the isolation procedure too daunting, and those who wish to improve their technique to produce more meaningful, reproducible data.

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Ronglih Liao, Ph.D.

Professor of Medicine
Stanford University School of Medicine

Dr. Ronglih Liao is a Professor of Medicine at Stanford University School of Medicine, co-Director of the Stanford Amyloid Center, and visiting Professor of Medicine at Brigham and Women’s Hospital, Harvard Medical School. As a world-renowned expert in cardiac dysfunction, she has served in numerous leadership roles in international societies, committees, and funding agencies. With direct funding from the NIH, Dr. Liao continues her groundbreaking research, and has made critical contributions to the understanding of the molecular mechanisms underlying the development of amyloid cardiomyopathy as well as the regulation of cell fate and function in adult cardiac progenitor cells.

Davor Pavlovic, Ph.D.

Lecturer in Cardiovascular Sciences
College of Medical and Dental Sciences
University of Birmingham

Dr. Davor Pavlovic is a Lecturer in Cardiovascular Sciences within the Institute of Cardiovascular Sciences at the University of Birmingham. His primary research interest is in understanding the molecular mechanisms of ion transport in the heart and how these may be targeted to treat cardiovascular disease. Dr. Pavlovic has published a number of research papers and review articles focused on the regulation of ion homeostasis in normal physiology and disease, particularly heart failure, arrhythmia and cardiorenal syndrome. Davor’s work is funded by the Wellcome Trust, Medical Research Council and British Heart Foundation, and has been acknowledged by awards by the International Society for Heart Research and the European Society of Cardiology.