A scientific discussion of the advancements in rodent surgical monitoring

On January 15, 2014, InsideScientific and Indus Instruments hosted a webinar about the importance of multimetric surgical monitoring to ensure survival rate and more consistent results. Speaker Graham Sattler of Indus Instruments discussed essentials of surgical monitoring and described the signal integration of the Mouse Monitor S. Next, Dr. Corey Reynolds outlined NIH guidelines and requirements, highlighted the importance of integrated surgical monitoring systems, and describes applications in the Mouse Phenotyping Core. Dr. Merry Lindsey then discussed applications in the lab, using as an example her research in cardiac remodelling following myocardial infarction.

Follow the links below to access key educational points of the webinar…

Graham Sattler

  • 05:00  Surgical monitoring overview, objectives, and outcomes
  • 07:39  Essentials of surgical monitoring
  • 09:16  Critical surgical monitoring signals and signal integration

Dr. Corey Reynolds

  • 16:20  Mouse Phenotyping Core background and services
  • 17:18  Challenges and requirements for a core facility
  • 20:26  Advantages of integrated monitoring and recording
  • 21:42  Applications demonstration

Dr. Merry Lindsey

  • 26:08  Myocardial infarction (MI) and post-MI cardiac remodelling: current research areas
  • 31:35  Example mouse recording for a pre- and post-MI setting
  • 33:20  Critical needs and best practices for in vivo models and surgery

Q&A

  • 37:16  Are there other research models this could be used for?
  • 38:28  Can the Mouse Monitor S be integrated with other PV loop systems?
  • 40:28  In the event someone has a lab with multiple platforms, how would you suggest arranging that?
  • 43:02  Can you describe the process and the options available through the Mouse Monitor directly for ECG filtering?
  • 46:00  Could a signal be left ‘untouched’ so it could be passed on to an external data acquisition system so data management could be handled downstream?
  • 47:08  Could you talk a bit about the pulse oximetry module? What system features have been built in to ensure optimal signal clarity, because there are so many signals pulled to one device?

SPEAKERS:

REFERENCES & CITATIONS:

  1. Guide for the care and use of laboratory animals. (2011). Washington, D.C.: National Academies Press.
  2. Thygesen, K., Alpert, J. S., & White, H. D. (2007). Universal Definition of Myocardial Infarction. Journal of the American College of Cardiology, 50(22), 2173-2195. doi:10.1016/j.jacc.2007.09.011
  3. Yabluchanskiy, A., Chilton, R. J., & Lindsey, M. L. (2013). Left ventricular remodeling: one small step for the extracellular matrix will translate to a giant leap for the myocardium. Congestive Heart Failure (Greenwich, Conn.), 19(4), E5–E8. http://doi.org/10.1111/chf.12023
  4. Lindsey, M. L., Escobar, G. P., Dobrucki, L. W., Goshorn, D. K., Bouges, S., Mingoia, J. T., . . . Spinale, F. G. (2006). Matrix metalloproteinase-9 gene deletion facilitates angiogenesis after myocardial infarction. American Journal of Physiology-Heart and Circulatory Physiology, 290(1). doi:10.1152/ajpheart.00457.2005
  5. Chiao, Y. A., Zamilpa, R., Lopez, E. F., Dai, Q., Escobar, G. P., Hakala, K., … Lindsey, M. L. (2010). In vivo Matrix Metalloproteinase-7 Substrates Identified in the Left Ventricle Post-Myocardial Infarction Using Proteomics. Journal of Proteome Research, 9(5), 2649–2657. http://doi.org/10.1021/pr100147r
  6. Ma, Y., Halade, G. V., Zhang, J., Ramirez, T. A., Levin, D., Voorhees, A., … Lindsey, M. L. (2013). Matrix Metalloproteinase-28 Deletion Exacerbates Cardiac Dysfunction and Rupture Following Myocardial Infarction in Mice by Inhibiting M2 Macrophage Activation. Circulation Research, 112(4), 675–688. http://doi.org/10.1161/CIRCRESAHA.111.300502

Author:

Liam Sanio

Account Manager, InsideScientific
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