Join Dr. Barbara Lind and Dr. Adrián Rodríguez-Contreras as they discuss neurovascular coupling in awake head-fixed and anesthetized mice, with a focus on the effects of anesthetics.

Please Note: The recording of this webinar is not yet available but will be published in early 2023 – register to be notified when on-demand access is enabled.

Neurovascular coupling (NVC) modulates cerebral blood flow to match increased metabolic demand during neuronal excitation. While we know that astrocyte calcium levels rise with excitatory neurotransmission, much less is understood about astrocytic sensitivity to inhibitory neurotransmission. In addition, general anesthetics, widely used in both clinical practice and preclinical research, have been shown to cause large disruptions in cerebral blood flow, brain metabolism, NVC, and functional connectivity. This suggests that they may have direct and indirect effects on the physiology of cerebrovascular endothelial cells (CEC).

In this webinar, Dr. Barbara Lind (Assistant Professor at the University of Copenhagen) and Dr. Adrián Rodríguez-Contreras (Associate Professor at Northwestern University) try to fill some of our knowledge gaps concerning NVC. Their insights stem from recent two-photon imaging and optogenetics studies performed in awake head-fixed and anesthetized mice. The presentations will elucidate the roles of astrocytes, interneurons, CECs, and calcium signaling in vivo, and specifically the effects of anesthesia on NVC.

Key Topics Include:

  • Advantages, limitations, and important considerations for using optical methods to investigate neurovascular physiology in animal models
  • The effects of general anesthetics on cerebrovascular functions, including the diversity in astrocytic calcium responses to interneuronal activation
  • Advantages of studying NVC in awake head-fixed mice and the available solutions
  • Hypotheses that explain cerebrovascular responses to experimental manipulations
  • Astrocytic responses to parvalbumin interneuron activation and their contribution to GABA-mediated NVC

Presenters

Associate Professor
Communication Sciences and Disorders
Northwestern University

Adrián Rodríguez-Contreras is an Associate Professor and director of the Auditory Development Laboratory at the Roxelyn and Richard Pepper Department of Communication Sciences and Disorders in the School of Communication, Northwestern University.

Assistant Professor
Department of Neuroscience
University of Copenhagen

Assist. Prof. Barbara Lykke Lind has recently set up her own lab in the Department of Neuroscience at the University of Copenhagen. In recent years she has worked on a project on astrocytic responses and blood flow regulation in the cortex of mice with chronically implanted cranial windows.

Production Partner

Neurotar

Neurotar develops instrumentation for microscopic imaging and electrophysiological recordings in the brain of awake head-fixed mice. Its proprietary Mobile HomeCage® allows integrating high-precision tests with behavior, starting from simple locomotion tracking to maze navigation. Neurotar has extensive imaging experience: the company pioneered in vivo two-photon imaging as contract research for the pharmaceutical industry in 2010. It has since extended its service portfolio to imaging studies in awake behaving mice.

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