Dr. Francesco Berti describes the use of proton NMR for structural identity and conformity testing of carbohydrate-based vaccines.
Polysaccharide-protein conjugate vaccines are among the safest and most successful vaccines developed in the last 30 years. Since the first semisynthetic chemical conjugate vaccine was licensed in the 1980’s to protect human against Haemophilus influenzae type b infection, conjugate vaccines against Neisseria meningitidis and Streptococcus pneumoniae have been developed and registered using the same approach (i.e. bacterial growth to produce capsular polysaccharide antigen and chemical coupling to carrier protein).
Physicochemical techniques are a powerful tool for the structural characterization of those vaccines. High-field Nuclear Magnetic Resonance (NMR) spectroscopy has been established as an extremely useful and robust method for tracking the industrial manufacturing process of these vaccines from polysaccharide bulk antigen through to the final formulation.
In this Bruker webinar, Dr. Francesco Berti will describe the use of proton NMR for structural identity and conformity testing of carbohydrate-based vaccines.
Scientific Director, Technical R&D