Dr. Reilly Enos and Dr. Eran Levin discuss estrogen's metabolic impact and how isotopic labeling and 13C-labeled nutrients can be used for animal physiology and nutrition research.

Reilly Enos, PhD – Harnessing the power of estrogen to regulate metabolic processes
Dr. Reilly Enos’ research focuses on the role that sex steroids and their receptors play in regulating metabolic processes, particularly in the setting of obesity. In this webinar, Dr. Enos will discuss his research on tissue-specific fluctuations of sex steroids throughout the estrous cycle in mice, provide insights into the importance of the quantity of estrogen necessary to impact physiological processes, as well as an understanding of the central versus peripheral effects of estrogen action.

Eran Levin, PhD – Unlocking Insights: Utilizing 13C Labeled Nutrients for Cutting-Edge Physiology and Nutrition Research
Dr. Eran Levin will discuss the potential of using 13C-labeled nutrients in physiology and nutrition research in animal models. Specifically, he will share practical tips for designing and conducting experiments using isotopic labeling techniques and demonstrate how they can provide unprecedented insights into metabolic pathways, nutrient utilization, and behaviors in both vertebrate and invertebrate models including insects, reptiles, and mammals.

Key Topics Include:

  • The role that estrogen plays in regulating metabolic and behavioral processes in males and females
  • The tissue-specific fluctuations of sex steroids throughout the estrous cycle
  • Insight into the importance of tissue-specificity in developing hormonal therapies
  • The importance of estrogen quantity in regulating physiological processes
  • Understand the diverse range of 13C labeled nutrients available
  • Specific applications of labeled amino acids in studies of protein metabolism, cellular signaling, and typical nutrient utilization
  • How to integrate 13C labeling techniques with respirometry for a comprehensive assessment of metabolic processes, energy expenditure, and substrate utilization in animal models
  • How to calculate metabolic rates in free-flying animals using 13C bicarbonate


Assistant Professor
Pathology, Microbiology, Immunology
University of South Carolina, School of Medicine

Dr. Enos is an Assistant Professor at the University of South Carolina, School of Medicine. Dr. Enos received his BA and BS degrees from the College of Wooster and the University of Northern Colorado, respectively and an MS from Baylor University. Dr. Enos earned his PhD from the University of South Carolina in Exercise Science (Applied Physiology) - the number one ranked kinesiology doctoral program in the United States. The Enos Lab is focused on understanding the molecular mechanisms regulating sex differences in obesity, particularly with respect to metabolic and inflammatory processes. A particular emphasis is the role that sex steroids and their tissue-specific receptors have in regulating glucose and fatty acid metabolism. Additionally, the lab is interested in exploring complementary and alternative therapies for the treatment of menopause-induced side effects, including excess fat mass accrual as well as metabolic and inflammatory perturbations. The laboratory is equipped with state-of-the-art equipment in order to perform metabolic phenotyping of rodent models. To date, Dr. Enos has published over 50 peer-reviewed publications and is currently funded by an NIH K01 Career Development Award.

Head of the Nutritional Ecology Lab
School of Zoology
Tel-Aviv University

In his research, Dr. Eran Levin studies the links between proximate physiological mechanisms and ultimate life-history evolution through a lens of resource allocation and nutrition. He is focused on nutrition, as it impinges on virtually all aspects of an animal’s life and is central to many interactions between an organism, its form and function, its environment, or other organisms. As most metabolic pathways of macronutrients are conserved across all animals, it allows Dr. Levin to choose the appropriate model best suited to address specific questions of metabolic adaptation, integrating ecological, behavioral, physiological, biochemical, and endocrinal scales and levels of the organization.

Production Partner

Sable Systems International Inc.

Sable Systems International contributes to the research community with superior instrumentation and software for innovation and discovery. Their metabolic phenotyping systems measure calorimetry, respirometry, metabolic/behavioral phenotyping and gas analysis at the best possible resolution and precision, providing unprecedented analytical and statistical power.

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