In this webinar, Dr. Conor McCartin discusses how phage display can be used for human antibody development.
While Phage Display panning on naïve libraries combined with costly affinity maturation strategies have allowed the successful development of neutralizing antibodies, it also leads to failures and aborted projects. One reason for this is the immune tolerance of a naïve library that is sometimes ineffective at identifying binders against important and/or highly expressed human targets. An efficient way to access high-affinity, fully human antibodies against such targets is thus essential for human cancer therapy.
In this webinar, Conor will discuss innovative technologies to develop such antibodies using human phage display. These strategies include:
- A cross-panning protocol for a successful identification of neutralizing binders.
- Phage display libraries to allow an efficient identification of neutralizing binders through competitive ELISA.
- A new human cancer library constructed from the PBMCs of cancer patients as an efficient solution to counter immune tolerance.
Key Topics Include:
- Efficient identification of neutralizing antibodies
- Strategic development of human phage libraries to break immune tolerance
- Strategies to augment phage display-based discovery
- Categories: Cancer & Oncology Research, Cell & Molecular Biology, Clinical Research, Drug Discovery & Development, Microbiology & Immunology
- Tags: antibody generation, antibody library, phage display
Monoclonal Antibody Development