Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal

Join Pilhan Kim, PhD for an in-depth discussion on the All-in-One intravital two-photon and confocal microscopy system.

Intravital microscopy is a unique imaging technique to visualize various in vivo cellular-level dynamics such as cell trafficking, cell-to-cell, or cell-to-microenvironment interactions in a live animal. Intravital imaging of cellular dynamics in a natural physiological microenvironment can provide unprecedented insights into the dynamic pathophysiology of human diseases that were impossible to obtain through conventional histological observation of ex vivo samples or in vitro culture systems. It is a unique tool for the development of new therapeutics and diagnostics by providing improved accuracy and reliability for in vivo target validation with delivery monitoring and efficacy assessment. It has been used to directly analyze the delivery and efficacy of new biopharmaceuticals such as antibodies, cell therapy, gene therapy, nucleic acids, and exosomes in an in vivo microenvironment.

The All-in-One intravital microscopy system can acquire real-time, multi-color, sub-micron resolution microscopic images in a live animal model with automatic motion compensation, enabling direct imaging analysis of complex cancer immune-microenvironments consisting of various immune cells, stromal cells, vascular cells, and extracellular matrix. In this talk, intravital imaging of various organs is touched upon. Additionally, recent studies utilizing the real-time intravital imaging technique are presented.