Join Michael Czech, PhD as he discusses adipose tissue "browning" as a treatment for metabolic disease, and presents recent data using light sheet microscopy to visualize functional changes in adipose tissue depots.

Adipose tissue is central to metabolic health and its disruption in obesity and type 2 diabetes causes havoc with normal body processes. In lean people, white adipocytes sequester fat away from other tissues where it can be toxic, while brown or beige adipocytes rapidly remove fat by metabolic oxidation. In addition, healthy white, beige and brown adipocytes act as endocrine cells by secreting factors beneficial to other tissues. However, pharmaceutical approaches to restore these therapeutic features of adipose tissue function in obesity and diabetes have had limited success.

In this presentation, Michael Czech, PhD shows how imaging adipose tissue depots from mice under various conditions by light sheet microscopy has illuminated changes in their function. Data are presented on the advancement of a novel CRISPR-based approach to enhance the “browning” and therapeutic efficacy of human adipocytes when implanted into humanized mice. Analysis of adipose implants by light sheet microscopy also adds to the wealth of data obtained on this cell therapy approach to alleviate obesity and type 2 diabetes.

Dr. Czech’s manuscript can be viewed on bioRxiv: https://www.biorxiv.org/content/10.1101/2020.10.13.337923v1

Key Topics Include:

  • Visualization of adipose tissue cell compositions and morphology in 3D by light sheet microscopy
  • Understanding how adipose tissue functions to control whole body metabolism
  • Learning CRISPR methods for gene modification in adipocytes with high efficiency
  • Learning methods of adipocyte implantation in “humanized” mice to alleviate diabetes

Click to watch the webinar recording. To view the presentation full screen simply click the square icon located in the bottom-right corner of the video viewer.

Resources

Presenters

Michael Czech, ;PhD

Michael Czech, PhD

Professor
Molecular Medicine
University of Massachusetts Medical School

Michael P. Czech is the Isadore and Fannie Foxman Professor at the University of Massachusetts Medical School, where he was founding Chair of the Program in Molecular Medicine (1989-2018). He has received the Elliot P. Joslin Medal, the Banting Medal of the ADA, and the Jacobaeus Prize for his work on adipose tissue metabolism in diabetes.

Production Partner

Miltenyi Biotec

Empowering cell research. Enabling cell therapy. For more than 30 years, Miltenyi Biotec has played an important role in the design, development, manufacture, and integration of products that empower the advancement of biomedical research and enable cell and gene therapy.

VIEW PROFILE
Miltenyi Biotec

Additional Content From Miltenyi Biotec

Remodeling of Pancreatic Innervation in Diabetes

Remodeling of Pancreatic Innervation in Diabetes

Dr. Sarah Stanley and Dr. Alexandra Alvarsson discuss the use of whole-organ assessment for the comprehensive examination of pancreatic innervation, revealing close interactions between nerves and islets and dynamic regulation of islet innervation in diabetes.

Additional Content From American Physiological Society

Related Content